Part:BBa_K1958007:Experience

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Binding residues of PbrR and its specificity

While finding the lead binding residues of PbrR, Part:BBa_K1958007 stated that that Cys78, Cys113 and Cys122 lead binding residues PbrR, however, these are the binding residues of its recombinant homologue PbrR691 [2]. On translating the DNA sequence attached with the part, it was found that the translated amino acid sequence is of PbrR which has no Cys residues at positions 78, 113, and 122. The Cys residues of PbrR are at positions 79, 114 and 123 which are lead-binding residues of PbrR as mentioned in literature [1].

The part originally also stated that PbrR has 1000-fold greater specificity for Pb that other heavy metals. However, this is not the case for PbrR, rather it is true for PbrR691.

References

[1] https://doi.org/10.1007/s10529-018-2533-4
[2] https://doi.org/10.1515%2F9783110434330-010